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The character of the periosteum is differentwhere ligaments and tendons attach to the bone.Collagen fibers from these structures extend obliquelyor at right angles to the long axis of the bone, wherethey are continuous with the collagen fibers of the ex-tracellular matrix. The tissue was also stained redwith antibody against smooth muscle a-actin conjugated directly with CY3fluorescent dye

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The stages of melanoma were classified on levels, whichwere associated with the thickness of melanoma lesions (level I = thinnest, level V = thickest)and based on a micro staging classification system developed by Dr.

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